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rabbit anti ryr3 antibody  (Alomone Labs)


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    Alomone Labs rabbit anti ryr3 antibody
    Effect of blocking L-type calcium channels with nifedipine and <t>ryanodine</t> <t>receptors</t> with dantrolene on ATP and BzATP-induced peak [Ca 2+ ] i . Cultured rat conjunctival goblet cells were preincubated with nifedipine (Nif) (10 −5 M) for 15 min and/or dantrolene (Da) (10 −5 M) for 30 min before stimulation with ATP (10 −5 M) ( A ) or BzATP (10 −4 M) ( B ). A and B : the average [Ca 2+ ] i over time. Arrows represent the addition of agonists. C : the peak increase of [Ca 2+ ] i above baseline. ATP: bars to the left of the central vertical dotted line in C , BzATP: bars to the right of the central vertical dotted line in C . Data are presented as means ± SE. n = 3. BzATP, benzoylbenzoyl-ATP; [Ca 2+ ] i , intracellular calcium concentration. *Statistically significant difference from agonist alone.
    Rabbit Anti Ryr3 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+ryr3+antibody/pmc11427011-51-16-19?v=Alomone+Labs
    Average 92 stars, based on 8 article reviews
    rabbit anti ryr3 antibody - by Bioz Stars, 2026-08
    92/100 stars

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    1) Product Images from "Purinergic agonists increase [Ca 2+ ] i in rat conjunctival goblet cells through ryanodine receptor type 3"

    Article Title: Purinergic agonists increase [Ca 2+ ] i in rat conjunctival goblet cells through ryanodine receptor type 3

    Journal: American Journal of Physiology - Cell Physiology

    doi: 10.1152/ajpcell.00291.2024

    Effect of blocking L-type calcium channels with nifedipine and ryanodine receptors with dantrolene on ATP and BzATP-induced peak [Ca 2+ ] i . Cultured rat conjunctival goblet cells were preincubated with nifedipine (Nif) (10 −5 M) for 15 min and/or dantrolene (Da) (10 −5 M) for 30 min before stimulation with ATP (10 −5 M) ( A ) or BzATP (10 −4 M) ( B ). A and B : the average [Ca 2+ ] i over time. Arrows represent the addition of agonists. C : the peak increase of [Ca 2+ ] i above baseline. ATP: bars to the left of the central vertical dotted line in C , BzATP: bars to the right of the central vertical dotted line in C . Data are presented as means ± SE. n = 3. BzATP, benzoylbenzoyl-ATP; [Ca 2+ ] i , intracellular calcium concentration. *Statistically significant difference from agonist alone.
    Figure Legend Snippet: Effect of blocking L-type calcium channels with nifedipine and ryanodine receptors with dantrolene on ATP and BzATP-induced peak [Ca 2+ ] i . Cultured rat conjunctival goblet cells were preincubated with nifedipine (Nif) (10 −5 M) for 15 min and/or dantrolene (Da) (10 −5 M) for 30 min before stimulation with ATP (10 −5 M) ( A ) or BzATP (10 −4 M) ( B ). A and B : the average [Ca 2+ ] i over time. Arrows represent the addition of agonists. C : the peak increase of [Ca 2+ ] i above baseline. ATP: bars to the left of the central vertical dotted line in C , BzATP: bars to the right of the central vertical dotted line in C . Data are presented as means ± SE. n = 3. BzATP, benzoylbenzoyl-ATP; [Ca 2+ ] i , intracellular calcium concentration. *Statistically significant difference from agonist alone.

    Techniques Used: Blocking Assay, Cell Culture, Concentration Assay

    RT-qPCR of the ryanodine receptor 3 (RyR3) subtype in rat and human conjunctival goblet cells (CGCs). Total RNA was isolated from rat and human CGCs and reverse-transcribed. cDNA amplification confirmed the presence of RyR3 mRNA in rat ( A ) but not in human ( B ) CGCs. Relative amount of RyR3 transcript compared with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was measured by quantitative PCR and is shown on y -axis. n = 3. RT-qPCR, quantitative reverse transcription polymerase chain reaction. *Statistically significant difference from 0.
    Figure Legend Snippet: RT-qPCR of the ryanodine receptor 3 (RyR3) subtype in rat and human conjunctival goblet cells (CGCs). Total RNA was isolated from rat and human CGCs and reverse-transcribed. cDNA amplification confirmed the presence of RyR3 mRNA in rat ( A ) but not in human ( B ) CGCs. Relative amount of RyR3 transcript compared with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was measured by quantitative PCR and is shown on y -axis. n = 3. RT-qPCR, quantitative reverse transcription polymerase chain reaction. *Statistically significant difference from 0.

    Techniques Used: Quantitative RT-PCR, Isolation, Reverse Transcription, Amplification, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction

    Proposed regulation of intracellular calcium levels by ryanodine receptor 3 (RyR3) in cultured rat conjunctival goblet cells. ATP and BzATP may increase [Ca 2+ ] i : 1 ) directly by activating RyR3, 2 ) by activating purinergic 1 (P1) receptors after the enzymatic conversion to metabolites, 3 ) by the activation of purinergic 2 (P2) receptors directly, and 4 ) by activating L-type calcium channels through membrane depolarization. These mechanisms can then either directly increase [Ca 2+ ] i through the influx of extracellular Ca 2+ and/or through the activation of RyR3 on an intracellular compartment such as the ER to release Ca 2+ . BzATP, benzoylbenzoyl-ATP; [Ca 2+ ] i , intracellular calcium concentration; CGCs, conjunctival goblet cells; CICR, calcium-induced calcium release.
    Figure Legend Snippet: Proposed regulation of intracellular calcium levels by ryanodine receptor 3 (RyR3) in cultured rat conjunctival goblet cells. ATP and BzATP may increase [Ca 2+ ] i : 1 ) directly by activating RyR3, 2 ) by activating purinergic 1 (P1) receptors after the enzymatic conversion to metabolites, 3 ) by the activation of purinergic 2 (P2) receptors directly, and 4 ) by activating L-type calcium channels through membrane depolarization. These mechanisms can then either directly increase [Ca 2+ ] i through the influx of extracellular Ca 2+ and/or through the activation of RyR3 on an intracellular compartment such as the ER to release Ca 2+ . BzATP, benzoylbenzoyl-ATP; [Ca 2+ ] i , intracellular calcium concentration; CGCs, conjunctival goblet cells; CICR, calcium-induced calcium release.

    Techniques Used: Cell Culture, Activation Assay, Membrane, Concentration Assay



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    Image Search Results


    Effect of blocking L-type calcium channels with nifedipine and ryanodine receptors with dantrolene on ATP and BzATP-induced peak [Ca 2+ ] i . Cultured rat conjunctival goblet cells were preincubated with nifedipine (Nif) (10 −5 M) for 15 min and/or dantrolene (Da) (10 −5 M) for 30 min before stimulation with ATP (10 −5 M) ( A ) or BzATP (10 −4 M) ( B ). A and B : the average [Ca 2+ ] i over time. Arrows represent the addition of agonists. C : the peak increase of [Ca 2+ ] i above baseline. ATP: bars to the left of the central vertical dotted line in C , BzATP: bars to the right of the central vertical dotted line in C . Data are presented as means ± SE. n = 3. BzATP, benzoylbenzoyl-ATP; [Ca 2+ ] i , intracellular calcium concentration. *Statistically significant difference from agonist alone.

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Purinergic agonists increase [Ca 2+ ] i in rat conjunctival goblet cells through ryanodine receptor type 3

    doi: 10.1152/ajpcell.00291.2024

    Figure Lengend Snippet: Effect of blocking L-type calcium channels with nifedipine and ryanodine receptors with dantrolene on ATP and BzATP-induced peak [Ca 2+ ] i . Cultured rat conjunctival goblet cells were preincubated with nifedipine (Nif) (10 −5 M) for 15 min and/or dantrolene (Da) (10 −5 M) for 30 min before stimulation with ATP (10 −5 M) ( A ) or BzATP (10 −4 M) ( B ). A and B : the average [Ca 2+ ] i over time. Arrows represent the addition of agonists. C : the peak increase of [Ca 2+ ] i above baseline. ATP: bars to the left of the central vertical dotted line in C , BzATP: bars to the right of the central vertical dotted line in C . Data are presented as means ± SE. n = 3. BzATP, benzoylbenzoyl-ATP; [Ca 2+ ] i , intracellular calcium concentration. *Statistically significant difference from agonist alone.

    Article Snippet: Cells were incubated with monoclonal mouse anti-RyR1/2 antibody (Thermo Fisher Scientific, Cat. No. MA3-916, RRID:AB_2183054) or rabbit anti-RyR3 antibody (Alomone Labs, Cat. No. ARR-003, RRID:AB_2040186) at a 1:50 or 1:100 dilution overnight at 4°C.

    Techniques: Blocking Assay, Cell Culture, Concentration Assay

    RT-qPCR of the ryanodine receptor 3 (RyR3) subtype in rat and human conjunctival goblet cells (CGCs). Total RNA was isolated from rat and human CGCs and reverse-transcribed. cDNA amplification confirmed the presence of RyR3 mRNA in rat ( A ) but not in human ( B ) CGCs. Relative amount of RyR3 transcript compared with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was measured by quantitative PCR and is shown on y -axis. n = 3. RT-qPCR, quantitative reverse transcription polymerase chain reaction. *Statistically significant difference from 0.

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Purinergic agonists increase [Ca 2+ ] i in rat conjunctival goblet cells through ryanodine receptor type 3

    doi: 10.1152/ajpcell.00291.2024

    Figure Lengend Snippet: RT-qPCR of the ryanodine receptor 3 (RyR3) subtype in rat and human conjunctival goblet cells (CGCs). Total RNA was isolated from rat and human CGCs and reverse-transcribed. cDNA amplification confirmed the presence of RyR3 mRNA in rat ( A ) but not in human ( B ) CGCs. Relative amount of RyR3 transcript compared with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was measured by quantitative PCR and is shown on y -axis. n = 3. RT-qPCR, quantitative reverse transcription polymerase chain reaction. *Statistically significant difference from 0.

    Article Snippet: Cells were incubated with monoclonal mouse anti-RyR1/2 antibody (Thermo Fisher Scientific, Cat. No. MA3-916, RRID:AB_2183054) or rabbit anti-RyR3 antibody (Alomone Labs, Cat. No. ARR-003, RRID:AB_2040186) at a 1:50 or 1:100 dilution overnight at 4°C.

    Techniques: Quantitative RT-PCR, Isolation, Reverse Transcription, Amplification, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction

    Proposed regulation of intracellular calcium levels by ryanodine receptor 3 (RyR3) in cultured rat conjunctival goblet cells. ATP and BzATP may increase [Ca 2+ ] i : 1 ) directly by activating RyR3, 2 ) by activating purinergic 1 (P1) receptors after the enzymatic conversion to metabolites, 3 ) by the activation of purinergic 2 (P2) receptors directly, and 4 ) by activating L-type calcium channels through membrane depolarization. These mechanisms can then either directly increase [Ca 2+ ] i through the influx of extracellular Ca 2+ and/or through the activation of RyR3 on an intracellular compartment such as the ER to release Ca 2+ . BzATP, benzoylbenzoyl-ATP; [Ca 2+ ] i , intracellular calcium concentration; CGCs, conjunctival goblet cells; CICR, calcium-induced calcium release.

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Purinergic agonists increase [Ca 2+ ] i in rat conjunctival goblet cells through ryanodine receptor type 3

    doi: 10.1152/ajpcell.00291.2024

    Figure Lengend Snippet: Proposed regulation of intracellular calcium levels by ryanodine receptor 3 (RyR3) in cultured rat conjunctival goblet cells. ATP and BzATP may increase [Ca 2+ ] i : 1 ) directly by activating RyR3, 2 ) by activating purinergic 1 (P1) receptors after the enzymatic conversion to metabolites, 3 ) by the activation of purinergic 2 (P2) receptors directly, and 4 ) by activating L-type calcium channels through membrane depolarization. These mechanisms can then either directly increase [Ca 2+ ] i through the influx of extracellular Ca 2+ and/or through the activation of RyR3 on an intracellular compartment such as the ER to release Ca 2+ . BzATP, benzoylbenzoyl-ATP; [Ca 2+ ] i , intracellular calcium concentration; CGCs, conjunctival goblet cells; CICR, calcium-induced calcium release.

    Article Snippet: Cells were incubated with monoclonal mouse anti-RyR1/2 antibody (Thermo Fisher Scientific, Cat. No. MA3-916, RRID:AB_2183054) or rabbit anti-RyR3 antibody (Alomone Labs, Cat. No. ARR-003, RRID:AB_2040186) at a 1:50 or 1:100 dilution overnight at 4°C.

    Techniques: Cell Culture, Activation Assay, Membrane, Concentration Assay

    AβOs injections decrease RyR2 immunolabeling. (A) Representative confocal microscopy 3-D projections (20 slices, 1.5 μM each) of CA1, CA3, and DG hippocampal regions isolated from food restricted rats exposed to the memory training protocol, fixed after the sixth training session and labeled with a RyR2 antibody (green) and DAPI-nuclear staining (blue). Scale bar: 20 μM. (B) Quantification of total RyR2 fluorescence normalized by total DAPI nuclear staining. Values are expressed as mean ± SE ( n = 6). Statistical analysis was determined by two-tailed unpaired Student’s t -test; ∗ p < 0.05.

    Journal: Frontiers in Aging Neuroscience

    Article Title: N-Acetylcysteine Prevents the Spatial Memory Deficits and the Redox-Dependent RyR2 Decrease Displayed by an Alzheimer’s Disease Rat Model

    doi: 10.3389/fnagi.2018.00399

    Figure Lengend Snippet: AβOs injections decrease RyR2 immunolabeling. (A) Representative confocal microscopy 3-D projections (20 slices, 1.5 μM each) of CA1, CA3, and DG hippocampal regions isolated from food restricted rats exposed to the memory training protocol, fixed after the sixth training session and labeled with a RyR2 antibody (green) and DAPI-nuclear staining (blue). Scale bar: 20 μM. (B) Quantification of total RyR2 fluorescence normalized by total DAPI nuclear staining. Values are expressed as mean ± SE ( n = 6). Statistical analysis was determined by two-tailed unpaired Student’s t -test; ∗ p < 0.05.

    Article Snippet: Primary antibodies: mouse monoclonal anti-RyR2 (MA3-916) was from Thermo Fisher Scientific (Waltham, MA, United States); rabbit monoclonal anti-RyR3 (AB9082) and rabbit polyclonal anti-IP 3 receptor type-1 (IP 3 R1) (AB5882) antibodies were from former Merck-Millipore (Darmstadt, Germany); rabbit polyclonal anti-ACSL4 (SAB2100035) and anti β-actin (A5316) were from Sigma-Aldrich (St. Louis, MI, United States); anti-VDAC (sc-390996), anti-Calnexin (sc-6465) and anti-COX4 (sc-69359) were from Santa Cruz Biotechnology (Dallas, TX, United States); rabbit monoclonal anti-β-amyloid (H31L21) was from Life Technologies (Waltham, MA, United States); rabbit c-Fos polyclonal antibody Ab-5 was from Oncogene (San Diego, CA, United States); Anti-Arc Polyclonal rabbit affinity purified antibody was from Synaptic System (Göttingen, Germany); ERK1/2 and phospho-ERK1/2 antibodies were from Cell Signaling Technologies (Danvers, MA, United States).

    Techniques: Immunolabeling, Confocal Microscopy, Isolation, Labeling, Staining, Fluorescence, Two Tailed Test

    AβOs-injections do not modify RyR3 immunolabeling. (A) Representative confocal microscopy 3-D projections (20 slices, 1.5 μM each) of CA1, CA3, and DG hippocampal regions from food restricted trained rats, fixed after the sixth training session and labeled with a RyR3 antibody (green) and DAPI staining for the nucleus (blue). Scale bar 20 μM. (B) Quantification of total fluorescence of RyR3 normalized by the total fluorescence of the DAPI nuclear staining. Statistical analysis was determined by two-tailed unpaired Student’s t -test.

    Journal: Frontiers in Aging Neuroscience

    Article Title: N-Acetylcysteine Prevents the Spatial Memory Deficits and the Redox-Dependent RyR2 Decrease Displayed by an Alzheimer’s Disease Rat Model

    doi: 10.3389/fnagi.2018.00399

    Figure Lengend Snippet: AβOs-injections do not modify RyR3 immunolabeling. (A) Representative confocal microscopy 3-D projections (20 slices, 1.5 μM each) of CA1, CA3, and DG hippocampal regions from food restricted trained rats, fixed after the sixth training session and labeled with a RyR3 antibody (green) and DAPI staining for the nucleus (blue). Scale bar 20 μM. (B) Quantification of total fluorescence of RyR3 normalized by the total fluorescence of the DAPI nuclear staining. Statistical analysis was determined by two-tailed unpaired Student’s t -test.

    Article Snippet: Primary antibodies: mouse monoclonal anti-RyR2 (MA3-916) was from Thermo Fisher Scientific (Waltham, MA, United States); rabbit monoclonal anti-RyR3 (AB9082) and rabbit polyclonal anti-IP 3 receptor type-1 (IP 3 R1) (AB5882) antibodies were from former Merck-Millipore (Darmstadt, Germany); rabbit polyclonal anti-ACSL4 (SAB2100035) and anti β-actin (A5316) were from Sigma-Aldrich (St. Louis, MI, United States); anti-VDAC (sc-390996), anti-Calnexin (sc-6465) and anti-COX4 (sc-69359) were from Santa Cruz Biotechnology (Dallas, TX, United States); rabbit monoclonal anti-β-amyloid (H31L21) was from Life Technologies (Waltham, MA, United States); rabbit c-Fos polyclonal antibody Ab-5 was from Oncogene (San Diego, CA, United States); Anti-Arc Polyclonal rabbit affinity purified antibody was from Synaptic System (Göttingen, Germany); ERK1/2 and phospho-ERK1/2 antibodies were from Cell Signaling Technologies (Danvers, MA, United States).

    Techniques: Immunolabeling, Confocal Microscopy, Labeling, Staining, Fluorescence, Two Tailed Test

    Hippocampal-derived MAM fractions contain RyR2, which is enriched in the MAM fraction from AβOs-injected rats. Western blot images of MAM fractions isolated from rat hippocampus (see Materials and Methods). The ER proteins analyzed were Calnexin (CNX), IP 3 R1, and RyR2. The inner and outer mitochondrial membrane proteins analyzed were COX1 and VDAC1, respectively; the characteristic MAM protein was ACSL-4. C, control, saline-injected; AβOs, AβOs-injected. The column at right indicates the ratios between band densities of each MAM protein (AβOs/control) normalized to b-tubulin protein levels.

    Journal: Frontiers in Aging Neuroscience

    Article Title: N-Acetylcysteine Prevents the Spatial Memory Deficits and the Redox-Dependent RyR2 Decrease Displayed by an Alzheimer’s Disease Rat Model

    doi: 10.3389/fnagi.2018.00399

    Figure Lengend Snippet: Hippocampal-derived MAM fractions contain RyR2, which is enriched in the MAM fraction from AβOs-injected rats. Western blot images of MAM fractions isolated from rat hippocampus (see Materials and Methods). The ER proteins analyzed were Calnexin (CNX), IP 3 R1, and RyR2. The inner and outer mitochondrial membrane proteins analyzed were COX1 and VDAC1, respectively; the characteristic MAM protein was ACSL-4. C, control, saline-injected; AβOs, AβOs-injected. The column at right indicates the ratios between band densities of each MAM protein (AβOs/control) normalized to b-tubulin protein levels.

    Article Snippet: Primary antibodies: mouse monoclonal anti-RyR2 (MA3-916) was from Thermo Fisher Scientific (Waltham, MA, United States); rabbit monoclonal anti-RyR3 (AB9082) and rabbit polyclonal anti-IP 3 receptor type-1 (IP 3 R1) (AB5882) antibodies were from former Merck-Millipore (Darmstadt, Germany); rabbit polyclonal anti-ACSL4 (SAB2100035) and anti β-actin (A5316) were from Sigma-Aldrich (St. Louis, MI, United States); anti-VDAC (sc-390996), anti-Calnexin (sc-6465) and anti-COX4 (sc-69359) were from Santa Cruz Biotechnology (Dallas, TX, United States); rabbit monoclonal anti-β-amyloid (H31L21) was from Life Technologies (Waltham, MA, United States); rabbit c-Fos polyclonal antibody Ab-5 was from Oncogene (San Diego, CA, United States); Anti-Arc Polyclonal rabbit affinity purified antibody was from Synaptic System (Göttingen, Germany); ERK1/2 and phospho-ERK1/2 antibodies were from Cell Signaling Technologies (Danvers, MA, United States).

    Techniques: Derivative Assay, Injection, Western Blot, Isolation

    NAC feeding prevents the RyR2 downregulation induced by AβOs, without modifying RyR3 protein levels. The hippocampus of water deprived, spatial memory trained rats belonging to the following groups was removed and homogenized 1 h after the last training session. The four groups were: vehicle-fed rats injected with saline (control), vehicle-fed rats injected with AβOs (AβOs), NAC-fed rats injected with AβOs (NAC/AβOs), and NAC-fed rats injected with saline (NAC/Saline). (A) . Representative Western blot and relative quantification of RyR2 protein bands normalized to β-actin and expressed as fold of control. (B) Representative Western blot and relative quantification of RyR3 protein bands normalized to β-actin and expressed as fold of control. Values are expressed as mean ± SE ( n = 3, for each group). Statistical analysis was determined by one-way ANOVA followed by Holm-Sidak post hoc test; ∗ p < 0.05 respect to control; ## p < 0.01 respect to AβOs.

    Journal: Frontiers in Aging Neuroscience

    Article Title: N-Acetylcysteine Prevents the Spatial Memory Deficits and the Redox-Dependent RyR2 Decrease Displayed by an Alzheimer’s Disease Rat Model

    doi: 10.3389/fnagi.2018.00399

    Figure Lengend Snippet: NAC feeding prevents the RyR2 downregulation induced by AβOs, without modifying RyR3 protein levels. The hippocampus of water deprived, spatial memory trained rats belonging to the following groups was removed and homogenized 1 h after the last training session. The four groups were: vehicle-fed rats injected with saline (control), vehicle-fed rats injected with AβOs (AβOs), NAC-fed rats injected with AβOs (NAC/AβOs), and NAC-fed rats injected with saline (NAC/Saline). (A) . Representative Western blot and relative quantification of RyR2 protein bands normalized to β-actin and expressed as fold of control. (B) Representative Western blot and relative quantification of RyR3 protein bands normalized to β-actin and expressed as fold of control. Values are expressed as mean ± SE ( n = 3, for each group). Statistical analysis was determined by one-way ANOVA followed by Holm-Sidak post hoc test; ∗ p < 0.05 respect to control; ## p < 0.01 respect to AβOs.

    Article Snippet: Primary antibodies: mouse monoclonal anti-RyR2 (MA3-916) was from Thermo Fisher Scientific (Waltham, MA, United States); rabbit monoclonal anti-RyR3 (AB9082) and rabbit polyclonal anti-IP 3 receptor type-1 (IP 3 R1) (AB5882) antibodies were from former Merck-Millipore (Darmstadt, Germany); rabbit polyclonal anti-ACSL4 (SAB2100035) and anti β-actin (A5316) were from Sigma-Aldrich (St. Louis, MI, United States); anti-VDAC (sc-390996), anti-Calnexin (sc-6465) and anti-COX4 (sc-69359) were from Santa Cruz Biotechnology (Dallas, TX, United States); rabbit monoclonal anti-β-amyloid (H31L21) was from Life Technologies (Waltham, MA, United States); rabbit c-Fos polyclonal antibody Ab-5 was from Oncogene (San Diego, CA, United States); Anti-Arc Polyclonal rabbit affinity purified antibody was from Synaptic System (Göttingen, Germany); ERK1/2 and phospho-ERK1/2 antibodies were from Cell Signaling Technologies (Danvers, MA, United States).

    Techniques: Injection, Western Blot